Journal: Healthcare

Article Title: Isolation and Identification of Coliform Bacteria and Multidrug-Resistant Escherichia coli from Water Intended for Drug Compounding in Community Pharmacies in Jordan

doi: 10.3390/healthcare11030299

Figure Lengend Snippet: Colonies of Escherichia coli grown on m-Endo Agar LES, producing a dark-red metallic sheen.

Article Snippet: E. coli ATCC 8739 strain was included as a positive control.

Techniques:

Journal: Healthcare

Article Title: Isolation and Identification of Coliform Bacteria and Multidrug-Resistant Escherichia coli from Water Intended for Drug Compounding in Community Pharmacies in Jordan

doi: 10.3390/healthcare11030299

Figure Lengend Snippet: Total microbial and total coliform counts found in water samples used for reconstitution of drugs in Jordanian community pharmacies.

Article Snippet: E. coli ATCC 8739 strain was included as a positive control.

Techniques:

Journal: Healthcare

Article Title: Isolation and Identification of Coliform Bacteria and Multidrug-Resistant Escherichia coli from Water Intended for Drug Compounding in Community Pharmacies in Jordan

doi: 10.3390/healthcare11030299

Figure Lengend Snippet: Summary statistics on source of water in droppers used for pharmaceutical compounding in Jordanian community pharmacies. The table shows number of samples contaminated with Escherichia coli , mean (%) and standard error (SE). Statistical significance between source groups was inferred using Z-score and the p -values between the groups was reported.

Article Snippet: E. coli ATCC 8739 strain was included as a positive control.

Techniques:

Journal: Healthcare

Article Title: Isolation and Identification of Coliform Bacteria and Multidrug-Resistant Escherichia coli from Water Intended for Drug Compounding in Community Pharmacies in Jordan

doi: 10.3390/healthcare11030299

Figure Lengend Snippet: ( A ). The growth of Escherichia coli on MacConkey agar show colonies that are dry, donut shaped and dark pink in colour, surrounded with dark pink area of precipitated bile salts. ( B ). Growth of E. coli on EMB agar shows the characteristic green metallic sheen. ( C ). Morphology of E. coli cells by microscopy after gram staining, showing gram negative, short rods.

Article Snippet: E. coli ATCC 8739 strain was included as a positive control.

Techniques: Microscopy, Staining

Journal: Healthcare

Article Title: Isolation and Identification of Coliform Bacteria and Multidrug-Resistant Escherichia coli from Water Intended for Drug Compounding in Community Pharmacies in Jordan

doi: 10.3390/healthcare11030299

Figure Lengend Snippet: Resistance patterns of Escherichia coli isolates against the following antibiotics: doxycycline (DO, 30 mcg), ceftazidime (CAZ, 30 mcg), gentamicin (CN, 10 mcg), ciprofloxacin (CIP5, 5 mcg), azithromycin (AZM, 15 mcg), amoxicillin/clavulanic acid (AMC, 30 mcg), levofloxacin (LVX5, 5 mcg) and tetracycline (TE, 30 mcg).

Article Snippet: E. coli ATCC 8739 strain was included as a positive control.

Techniques:

Journal: Nanomaterials

Article Title: Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

doi: 10.3390/nano10102082

Figure Lengend Snippet: Antibacterial activities and minimum inhibitory concentrations (MICs) for Ag-NPs synthesized by S. antimycoticus L-1 against pathogenic S. aureus, B. subtilis, P. aeruginosa, E. coli, and S. typhimurium. # indicates MIC value.

Article Snippet: The antibacterial potential of nano-coated fabrics was assessed against bacterial pathogens: Gram-positive strains ( Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538) and Gram-negative strains ( Pseudomonas aeruginosa ATCC 9022 and Escherichia coli ATCC 8739).

Techniques: Synthesized

Journal: Nanomaterials

Article Title: Bactericidal and In-Vitro Cytotoxic Efficacy of Silver Nanoparticles (Ag-NPs) Fabricated by Endophytic Actinomycetes and Their Use as Coating for the Textile Fabrics

doi: 10.3390/nano10102082

Figure Lengend Snippet: Effect of repeated washing on the antibacterial properties of silver nanoparticle-finished fabrics by qualitative assessment method.

Article Snippet: The antibacterial potential of nano-coated fabrics was assessed against bacterial pathogens: Gram-positive strains ( Bacillus subtilis ATCC 6633 and Staphylococcus aureus ATCC 6538) and Gram-negative strains ( Pseudomonas aeruginosa ATCC 9022 and Escherichia coli ATCC 8739).

Techniques:

Journal: International Journal of Nanomedicine

Article Title: Green Synthesis of Zinc Oxide Nanoparticles as a Promising Nanomedicine Approach for Anticancer, Antibacterial, and Anti-Inflammatory Therapies

doi: 10.2147/IJN.S507214

Figure Lengend Snippet: Antibacterial Activity of CEB- ZnO-NPs

Article Snippet: The bacterial strains Escherichia coli ATCC 8739, Staphylococcus aureus ATCC 29213, Pseudomonas aeruginosa ATCC 9027, and Bacillus cereus ATCC 9634 were obtained from Nawah Scientific Inc. (Mokatam, Cairo, Egypt).

Techniques: Activity Assay

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Specificity and sensitivity of the IS900 nPCR in DNA extracts from the modified DNAzol ® and the standard phenol/chloroform/isoamyl-alcohol DNA extraction protocols. nPCR based on the IS900 specific to MAP was performed on DNA template extracted by the standard phenol/chloroform/isoamyl-alcohol DNA extraction (I) and the modified DNAzol ® DNA extraction technique (II). A 298 bp fragment on 2% agarose gel is positive for MAP. a (1) Non-pathogenic E. coli strain K-12; (2) S. aureus ; (3) L. monocytogenes ; (4) K. pneumoniae ; (5) M. smegmatis ; (6) M. avium subspecies avium ; (7) M. xenopi ; (8) M. fortuitum subspecies fortuitum ; (9) MAP Clinical Strain JF7. b (1) MAP Strain 1; (2) MAP Strain 3; (3) MAP Strain 8B; (4) MAP Para 18; (5) MAP UCF3; (6) MAP UCF5; (7) MAP UCF7; (8) MAP Linda; (9) MAP MS137. c Serial dilution of MAP UCF4 DNA concentrations were analyzed by nPCR. (1) 31.7 ng/μL; (2) 3.17 ng/μL; (3) 317 pg/μL; (4) 31.7 pg/μL; (5) 3.17 pg/μL; (6) 317 fg/μL; (7) 31.7 fg/μL; (8) 3.17 fg/μL; (9) 317 ag/μL; (10) 31.7 ag/μL. +: MAP UCF4; N: No DNA; M: molecular weight marker

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques: Modification, DNA Extraction, Agarose Gel Electrophoresis, Serial Dilution, Molecular Weight, Marker

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Validation of multiplex PCR using individual and combined oligonucleotide primer sets and DNA extracts from mixed bacterial culture. Multiplex PCR was performed on DNA extracts from mixed bacterial cultures. DNA template was extracted using the modified DNAzol ® . (1) Non-pathogenic E. coli strain K-12 18s primers were used (171 bp); (2) MAP UCF4 IS900 AV1/AV2 primers were used (298 bp); (3) K. pneumoniae 23s primers were used (493 bp); (4) Mycobacterium avium complex (MAC) IS1311 primers were used (534 bp); (5) a cocktail of the 4 primer sets mentioned above were used. M: DNA molecular weight marker

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques: Biomarker Discovery, Multiplex Assay, Modification, Molecular Weight, Marker

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Multiplex PCR and m -FISH results for intestinal tissue from IBD

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques: Multiplex Assay

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Validation of multiplex PCR using individual and combined oligonucleotide primer sets and DNA extracts from IBD tissue. Multiplex PCR was performed on DNA extracts from intestinal tissue samples. DNA template was extracted using the modified DNAzol ® . RS1: ulcerative colitis (UC) patient; RS2: Crohn’s disease (CD) patient; (1) Non-pathogenic E. coli strain K-12 18s primers were used (171 bp); (2) MAP UCF4 IS900 AV1/AV2 primers were used (298 bp); (3) AIEC strain LF82 g ipA primers were used (357 bp); (4) K. pneumoniae 23s primers were used (493 bp); (5) Mycobacterium avium complex (MAC) IS1311 primers were used (534 bp); (6) a cocktail of the 5 primer sets mentioned above were used. M: DNA molecular weight marker

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques: Biomarker Discovery, Multiplex Assay, Modification, Molecular Weight, Marker

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Gram stain, acid-fast stain and m -FISH detection of bacterial cultures. Gram stain (1), acid-fast stain (2), and oligonucleotide m -FISH (3) images of bacterial cultures: a , b Non-pathogenic E. coli strain K-12; c MAP UCF4; and d K. pneumoniae . For - m -FISH detection: a 3 m -FISH using non-pathogenic E. coli K-12 18s probe labeled with AF647 fluorophore; b 3 m -FISH using AIEC strain LF82 g ipA probe labeled with AF568 fluorophore; c 3 m -FISH using MAP UCF4 IS900 AV1 probe labeled with AF488 fluorophore; and d 3 m-FISH using K. pneumoniae 23s probe labeled with AF546 fluorophore. All microscopic images were obtained at 1000 × magnification. White measurement bar found in D3 represents 100 μm. All m -FISH images were obtained using CSLM

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques: Staining, Ziehl-Neelsen Stain, Labeling

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Use of m -FISH using individual probes to detect multiple pathogens in intestinal tissue. Oligonucleotide m -FISH analysis in tissue sections from patients with UC (RS1) and CD (RS2) was done targeting for: a Non-pathogenic E. coli strain K-12; b AIEC strain LF82; c MAP; and d K. pneumoniae . Images illustrating DAPI are in red. The individual m -FISH probes that were used were: a non-pathogenic E. coli strain K-12 18s probe labeled with AF647 fluorophore; b AIEC strain LF82 g ipA probe labeled with AF568 fluorophore; c MAP UCF4 IS900 AV1 probe labeled with AF488 fluorophore; and d K. pneumoniae 23s probe labeled with AF546 fluorophore. White measurement bars found in D1 and D2 represents 20 μm. All m -FISH images were obtained using CSLM

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques: Labeling

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Use of m -FISH using combined probes to detect multiple pathogens in intestinal tissue from CD. Oligonucleotide m -FISH images of intestinal tissue sections from four CD patients ( a RS3, b RS4, c RS5, and d RS6). A combined probes mixture including (2) EC647 (non-pathogenic E. coli strain K-12 18s probe labeled with AF647 fluorophore) and (3) MAP488 (MAP UCF4 IS900 AV1 probe labeled with AF488 fluorophore) were used in all tissue sections. (1) DAPI staining and (4) merged images between DAPI and the corresponding probe. White measurement bar located in B3 represents 20 μm. All m -FISH images were obtained using CSLM

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques: Labeling, Staining

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Nucleotide primers used in multiplex PCR and m -FISH probes

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques: Multiplex Assay

Journal: Gut Pathogens

Article Title: Development of multiplex PCR and multi-color fluorescent in situ hybridization ( m -FISH) coupled protocol for detection and imaging of multi-pathogens involved in inflammatory bowel disease

doi: 10.1186/s13099-018-0278-1

Figure Lengend Snippet: Microorganisms used in this study

Article Snippet: Non - pathogenic Escherichia coli Strain K-12 , ATCC 8739.

Techniques:

Journal: 3 Biotech

Article Title: Engineering membrane morphology and manipulating synthesis for increased lycopene accumulation in Escherichia coli cell factories

doi: 10.1007/s13205-018-1298-8

Figure Lengend Snippet: Strains and plasmids used in this work

Article Snippet: Table 1 Strains and plasmids Relative characteristics Resources Strains CAR005 ATCC 8739, M1-37:: dxs , M1-46:: idi M1-93:: Crt , M1-46:: SucAB , M1-46:: sdh , M1-46:: talB Lab collection CAR015 CAR005, ispG-mRSL-4, ispH-mRSL-14 Lab collection CAR025 CAR015, replacing the promoter of crtEYIB with Ptrc promoter Lab collection LYC101 CAR025, △ crtY Lab collection LYC101-37Almgs LYC101-46Almgs LYC101, M1-37:: Almgs LYC101, M1-46:: Almgs This work This work Plasmids pACYC184-M cat; replace tet with lacI and Ptrc of pTrc99A-M Lab collection pCas9 Cas9 (Zhao et al. 2016 ) pAlmgs pACYC184-M with Ptrc controlled almgs This work pMtlA pACYC184-M with Ptrc controlled mtla This work pTsr pACYC184-M with Ptrc controlled tsr This work pPlsb pACYC184-M with Ptrc controlled plsb This work pPlsb-plsc pACYC184-M with Ptrc controlled plsb and plsc This work pPlsb-plsc-dgka pACYC184-M with Ptrc controlled plsb, plsc , and dgka This work Open in a separate window Strains and plasmids used in this work.

Techniques: